Stilbene Synthase and Chalcone Synthase
نویسندگان
چکیده
منابع مشابه
Cross-reaction of chalcone synthase and stilbene synthase overexpressed in Escherichia coli.
Chalcone synthase (CHS) and stilbene synthase (STS) are related plant polyketide synthases belonging to the CHS superfamily. CHS and STS catalyze common condensation reactions of p-coumaroyl-CoA and three C(2)-units from malonyl-CoA but different cyclization reactions to produce naringenin chalcone and resveratrol, respectively. Using purified Pueraria lobata CHS and Arachis hypogaea STS overex...
متن کاملStilbene Synthase and Chalcone Synthase : Two Different Constitutive Enzymes in Cultured Cells of Picea excelsa.
Cultured cells of Picea excelsa capable of forming stilbenes and flavanoids have been established. Unlike needles of intact plants containing piceatannol (3,3',4',5-tetrahydroxystilbene) and stilbene glycosides the cultured cells converted phenylalanine and p-coumaric acid primarily into resveratrol monomethyl ether (3,4'-dihydroxy-5-methoxystilbene) and naringenin. Partially purified enzyme pr...
متن کاملA comparison of stilbene and chalcone synthases including a new stilbene synthase gene from Vitis riparia cv. Gloire de Montpellier
A stilbene synthase gene was cloned from Vitis riparia cv. Gloire de Montpellier after PCR amplification with primers designed to include the start and stop codons of stilbene synthase genes of V. inifera. The exon was very similar to that of other stilbene synthases, particularly those from V. inifera (99% nucleotide identity). An intron was found which interrupted the predicted codon for cyst...
متن کاملTwo Different Chalcone Synthase Activities from Spinach
Chenopodiaceae, Spinacia oleracea, Flavonoid Metabolism, Chalcone Synthase, Two Different Activities Chalcone synthase activity was found in enzyme preparations from spinach. In homogenates o f young leaves two different activities o f the enzyme could be separated by DEAE-ion exchange chromatography and chromatofocusing. Both activities formed naringenin with [2-14C] malonylCoA and 4-coumaroyl...
متن کاملPurification of Chalcone Synthase from Tulip Anthers and Comparison with the Synthase from Cosmos Petals
Chalcone synthase was isolated from both anthers of Tulipa cv. “Apeldoorn” and petals of Cosmos sulphureus Cav. After certain prepurification steps, the enzymes were further purified using gel chromatography on Sephadex G-200 followed by repeated hydroxylapatite absorption chromatography. Both the enzymes showed the same chromatographic properties. After gel chromatography as well as after the ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1984
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.75.2.489